2021-06-02
I. Sterilization: use fresh, mold-free media in summer, filling and sterilizing bottles or bags promptly after mixing. Keep cotton plugs and kraft paper dry, and sterilize spare cotton and paper together in sealed plastic bags.
After sterilization, replace damp closures inside the inoculation box. Handle bags gently at every stage. Seal punctures or split bags with medical tape or an outer bag. Rubber bands or ties may replace cotton plugs and collars. Depending on substrate quantity, the article suggests extending bag sterilization by 1–2 hours and releasing steam slowly.
II. Inoculation: alcohol-lamp flames may raise inoculation-box temperatures to 40–50°C and injure spawn. Work during cooler mornings, evenings or nights, transfer fewer bottles at a time, use a somewhat larger inoculum and remove old mycelium from bottle mouths.
III. Keep incubation areas clean, free of pests and harmful substances, cool and out of direct sunlight, with relative humidity below 60%. Stack newly inoculated containers only after their surfaces and closures have dried. Set stack height and spacing for heat removal, ventilation and access. In summer, inspect about three days after inoculation and remove contaminated spawn promptly.
Hubei Changyin Edible Fungi Machinery Co., Ltd. supplies bag filling machines, clipping machines, dryers and other mushroom equipment to customers across China, supported by after-sales service.