2021-06-02
1. Maintain pure spawn cultures and avoid contaminated spawn.
2. Store spawn at an appropriately low temperature. Around 4°C helps preserve vigor in cool-growing mushrooms such as oyster and shiitake, while straw mushroom spawn is better kept near 16°C.
3. Avoid repeated transfers on a single medium or using spawn beyond its useful age. Aging and degeneration are related, and low-vigor spawn degenerates more readily.
4. Rejuvenate spawn regularly at a suitable temperature and pH, with adequate oxygen and clean culture conditions.
5. Use annual spore isolation to identify superior strains through sexual reproduction, and tissue isolation to stabilize their traits.
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