2021-06-02
Mutation breeding increases variation so that useful strains can be selected. Physical and chemical mutagens act directly or indirectly on nucleic acids. The archived source lists UV, X-rays, gamma rays, diethyl sulfate, 5-bromouracil, nitrogen mustard and nitrosoguanidine among examples; some names and abbreviations in the original are inconsistent.
Mutation breeding has three stages:
First, prepare a spore suspension by adding fresh sterile mushroom spores to 5 mL of sterile saline or phosphate buffer and mixing. The stated target concentration is 10^6–10^9 spores per mL.
Second, apply a mutagen such as UV light inside a dark box. The described setup uses a 15 W UV lamp 30 cm above the sample, warmed for 20 minutes, then exposes suspension in an open 6 cm sterile dish for 0.5–1 minute.
Third, screen the treated strains. Mutation increases variation without controlling its direction, so identifying useful individuals among many different variants is the most difficult stage.
Hubei Changyin Edible Fungi Machinery Co., Ltd. supplies bag filling machines, clipping machines, dryers and other mushroom equipment to customers across China, supported by after-sales service.